Search results for "Enterobacter aerogenes"
showing 7 items of 7 documents
Ūdeņraža mikrobioloģiska producēšana, izmantojot Enterobacter aerogenes, un uzkrāšana AB5 tipa sakausējumā
2017
Ūdeņradis ir atjaunojams un tīrs enerģijas nesējs, kuru var iegūt mikrobioloģiski fermentējot glicerīnu. Šī darba mērķis bija pētīt Enterobacter aerogenes spēju producēt ūdeņradi, fermentējot glicerīnu, kā arī hidrīdu veidojošu AB5 sakausējumu spēju uzkrāt ūdeņradi, tiem atrodoties barotnē ar baktērijām. Barotņu gāzes fāzes sastāvu noteica ar masspektrometru un pārliecinājās par Enterobacter aerogenes spēju producēt ūdeņradi, fermentējot glicerīnu. Tika aprēķināts ūdeņraža produkcijas iznākums 0,15 moli H2 uz molu glicerīna. Konstatēja LaNi5 sakausējuma pulvera inhibējošo ietekmi uz Enterobacter aerogenes gāzu produkciju. Jaukta sastāva AB5 pulveris gāzu produkciju palielināja. Nosakot LaNi…
Impact of the Prone Position in an Animal Model of Unilateral Bacterial Pneumonia Undergoing Mechanical Ventilation
2013
Abstract Background: The prone position (PP) has proven beneficial in patients with severe lung injury subjected to mechanical ventilation (MV), especially in those with lobar involvement. We assessed the impact of PP on unilateral pneumonia in rabbits subjected to MV. Methods: After endobronchial challenge with Enterobacter aerogenes, adult rabbits were subjected to either “adverse” (peak inspiratory pressure = 30 cm H2O, zero end-expiratory pressure; n = 10) or “protective” (tidal volume = 8 ml/kg, 5 cm H2O positive end-expiratory pressure; n = 10) MV and then randomly kept supine or turned to the PP. Pneumonia was assessed 8 h later. Data are presented as median (interquartile range). Re…
Immunoblotting with Monoclonal Antibodies: A Highly Specific System for Detection and Identification of Bacterial Outer Membrane Proteins
1985
We are studying the E. coli outer membrane proteins traTp, ompAp, and “X”, a major outher membrane protein with a molecular weight of about 42000 daltons that has not yet been definitively identified, as potential targets for diagnostic reagents and vaccines.
D-Malic enzyme of Pseudomonas fluorescens.
1982
By the enrichment culture technique 14 gram-negative bacteria and two yeast strains were isolated that used D(+)-malic acid as sole carbon source. The bacteria were identified as Pseudomonas putida, Pseudomonas fluorescens, Pseudomonas aeruginosa and Klebsiella aerogenes. In cell-free extracts of P. fluorescens and P. putida the presence of malate dehydrogenase, D-malic enzyme (NAD-dependent) and L-malic enzyme (NADP-dependent) was demonstrated. D-Malic enzyme from P. fluorescens was purified. Stabilization of the enzyme by 50 mM ammonium sulphate an 1 mM EDTA was essential. Preparation of D-malic enzyme that gave one band with disc gel electrophoresis showed a specific activity of 4-5 U/mg…
Bacterial 2,3-butanediol dehydrogenases
1978
Enterobacter aerogenes, Aeromonas hydrophila, Serratia marcescens and Staphylococcus aureus possessing L(+)-butanediol dehydrogenase produced mainly meso-butanediol and small amounts of optically active butanediol; Acetobacter suboxydans, Bacillus polymyxa and Erwinia carotovora containing D(-)-butanediol dehydrogenase produced more optically active butanediol than meso-butanediol. Resting and growing cells of these organisms oxidezed only one enantiomer of racemic butanediol. The D(-)-butanediol dehydrogenase from Bacillus polymyxa was partially purified (30-fold) with a specific activity of 24.5. Except NAD and NADH no other cofactors were required. Optimum pH-values for oxidation and red…
Data from: Evolution of bacterial life history traits is sensitive to community structure
2016
Very few studies have experimentally assessed the evolutionary effects of species interactions within the same trophic level. Here we show that when Serratia marcescens evolve in multispecies communities, their growth rate exceeds the growth rate of the bacteria that evolved alone, whereas the biomass yield gets lower. In addition to the community effects per se, we found that few species in the communities caused strong effects on S. marcescens evolution. The results indicate that evolutionary responses (of a focal species) are different in communities, compared to species evolving alone. Moreover, selection can lead to very different outcomes depending on the community structure. Such con…
Data from: Propagule pressure increase and phylogenetic diversity decrease community’s susceptibility to invasion
2017
Invasions pose a large threat to native species, but the question of why some species are more invasive, and some communities more prone to invasions than others, is far from solved. Using ten different three-species bacterial communities, we tested experimentally if the phylogenetic relationships between an invader and a resident community and propagule pressure affect invasion probability. We found that greater diversity in phylogenetic distances between the resident community members and the invader lowered invasion success, and higher propagule pressure increased invasion success whereas phylogenetic distance had no clear effect. In the later stages of invasion phylogenetic diversity ha…